Pig vs Pig - Ossabaw miniature LastZ results
Pig (Sus scrofa, Sscrofa11.1) and Pig - Ossabaw miniature (Sus scrofa, ASM2471841v1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 113. Pig was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 5000 |
| Threshold for gapped extension (L) | 5000 |
| Threshold for alignments between gapped alignment blocks (H) | 3000 |
| Masking count (M) | 10 |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Pig | Pig - Ossabaw miniature |
|---|---|---|
| Chunk size | 10,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 10,100,000 | 10,100,000 |
| Masking options |
Statistics over 96,673 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Pig |
Uncovered: 54,862,752 out of 2,501,912,388 |
Uncovered: 352,361 out of 35,448,596 |
| Pig - Ossabaw miniature |
Uncovered: 62,134,577 out of 2,455,681,563 |
Uncovered: 845,209 out of 35,510,875 |
Block size distribution
| Size range | All 96,673 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 2,342 |
15.3 kb |
|||
| 10 bp - 100 bp | 12,842 |
608.2 kb |
2,928 |
177.9 kb |
|
| 100 bp - 1 kb | 26,375 |
10.4 Mb |
9,310 |
3.4 Mb |
|
| 1 kb - 10 kb | 22,913 |
87.0 Mb |
4,328 |
14.8 Mb |
|
| 10 kb - 100 kb | 25,038 |
966.1 Mb |
1,520 |
42.0 Mb |
|
| 100 kb - 1 Mb | 7,163 |
1.4 Gb |
90 |
21.8 Mb |
|
| 1 Mb - 10 Mb | 10 |
20.6 Mb |
|||
| 10 Mb - 100 Mb | 6 |
400.9 Mb |
|||
| 100 Mb - 1 Gb | 13 |
1.9 Gb |
|||