Mouse vs Steppe mouse LastZ results
Mouse (Mus musculus, GRCm39) and Steppe mouse (Mus spicilegus, MUSP714) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 103. Mouse was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Mouse | Steppe mouse |
|---|---|---|
| Chunk size | 10,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 10,100,000 | |
| Masking options |
Statistics over 599,731 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Mouse |
Uncovered: 299,689,216 out of 2,728,222,451 |
Uncovered: 210,036 out of 39,092,596 |
| Steppe mouse |
Uncovered: 237,873,011 out of 2,496,083,520 |
Uncovered: 812,468 out of 35,178,654 |
Block size distribution
| Size range | All 599,731 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 7,797 |
45.9 kb |
|||
| 10 bp - 100 bp | 68,897 |
3.5 Mb |
20,781 |
1.2 Mb |
|
| 100 bp - 1 kb | 165,745 |
72.0 Mb |
48,305 |
18.7 Mb |
|
| 1 kb - 10 kb | 284,619 |
1.1 Gb |
45,522 |
149.3 Mb |
|
| 10 kb - 100 kb | 72,661 |
1.2 Gb |
5,709 |
136.7 Mb |
|
| 100 kb - 1 Mb | 12 |
1.5 Mb |
1,204 |
460.5 Mb |
|
| 1 Mb - 10 Mb | 689 |
1.7 Gb |
|||
| 10 Mb - 100 Mb | 2 |
21.6 Mb |
|||