Human vs Blue whale LastZ results
Human (Homo sapiens, GRCh38) and Blue whale (Balaenoptera musculus, mBalMus1.v2) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 101. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Blue whale |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 10,100,000 | |
| Masking options |
Statistics over 2,061,539 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 1,480,608,425 out of 3,099,750,718 |
Uncovered: 1,629,363 out of 38,571,754 |
| Blue whale |
Uncovered: 859,256,416 out of 2,379,995,981 |
Uncovered: 914,988 out of 31,093,315 |
Block size distribution
| Size range | All 2,061,539 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 67,581 |
460.3 kb |
|||
| 10 bp - 100 bp | 266,948 |
12.7 Mb |
21,969 |
1.5 Mb |
|
| 100 bp - 1 kb | 1,148,797 |
520.9 Mb |
65,515 |
23.5 Mb |
|
| 1 kb - 10 kb | 576,694 |
1.2 Gb |
20,459 |
51.0 Mb |
|
| 10 kb - 100 kb | 1,503 |
22.3 Mb |
1,907 |
49.1 Mb |
|
| 100 kb - 1 Mb | 16 |
2.1 Mb |
260 |
64.4 Mb |
|
| 1 Mb - 10 Mb | 61 |
245.8 Mb |
|||
| 10 Mb - 100 Mb | 35 |
1.2 Gb |
|||
| 100 Mb - 1 Gb | 1 |
105.1 Mb |
|||