Human vs Brown trout LastZ results
Human (Homo sapiens, GRCh38) and Brown trout (Salmo trutta, fSalTru1.1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 99. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Brown trout |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 10,100,000 | |
| Masking options |
Statistics over 424,187 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 3,061,287,357 out of 3,099,750,718 |
Uncovered: 16,254,484 out of 38,571,754 |
| Brown trout |
Uncovered: 2,306,332,020 out of 2,371,880,186 |
Uncovered: 34,578,197 out of 79,685,618 |
Block size distribution
| Size range | All 424,187 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 3,141 |
14.0 kb |
|||
| 10 bp - 100 bp | 82,770 |
6.2 Mb |
27,803 |
2.1 Mb |
|
| 100 bp - 1 kb | 334,794 |
68.3 Mb |
146,743 |
43.4 Mb |
|
| 1 kb - 10 kb | 3,456 |
5.4 Mb |
16,132 |
31.6 Mb |
|
| 10 kb - 100 kb | 22 |
944.7 kb |
167 |
3.2 Mb |
|
| 100 kb - 1 Mb | 4 |
459.1 kb |
7 |
1.0 Mb |
|