Human vs Reedfish LastZ results
Human (Homo sapiens, GRCh38) and Reedfish (Erpetoichthys calabaricus, fErpCal1.1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 98. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Reedfish |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 10,100,000 | |
| Masking options | {default_soft_masking => 1} | {default_soft_masking => 1} |
Statistics over 209,868 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 3,058,586,268 out of 3,099,750,718 |
Uncovered: 15,961,372 out of 38,571,754 |
| Reedfish |
Uncovered: 3,780,979,435 out of 3,811,022,027 |
Uncovered: 17,159,589 out of 35,314,704 |
Block size distribution
| Size range | All 209,868 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 877 |
3.8 kb |
|||
| 10 bp - 100 bp | 36,488 |
2.8 Mb |
17,120 |
1.3 Mb |
|
| 100 bp - 1 kb | 169,993 |
36.9 Mb |
102,097 |
28.3 Mb |
|
| 1 kb - 10 kb | 2,489 |
3.8 Mb |
6,800 |
12.7 Mb |
|
| 10 kb - 100 kb | 20 |
471.5 kb |
73 |
1.3 Mb |
|
| 100 kb - 1 Mb | 1 |
139.5 kb |
3 |
350.6 kb |
|