Human vs Barramundi perch LastZ results
Human (Homo sapiens, GRCh38) and Barramundi perch (Lates calcarifer, ASB_HGAPassembly_v1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 97. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Barramundi perch |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 10,100,000 | |
| Masking options |
Statistics over 260,881 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 3,065,312,465 out of 3,099,750,718 |
Uncovered: 17,565,200 out of 38,571,754 |
| Barramundi perch |
Uncovered: 633,568,991 out of 668,464,831 |
Uncovered: 16,605,868 out of 41,392,853 |
Block size distribution
| Size range | All 260,881 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 1,841 |
8.3 kb |
|||
| 10 bp - 100 bp | 53,288 |
4.0 Mb |
14,931 |
1.1 Mb |
|
| 100 bp - 1 kb | 203,889 |
40.4 Mb |
79,549 |
24.2 Mb |
|
| 1 kb - 10 kb | 1,856 |
2.9 Mb |
10,401 |
20.7 Mb |
|
| 10 kb - 100 kb | 7 |
97.5 kb |
92 |
1.3 Mb |
|
| 100 kb - 1 Mb | 2 |
218.8 kb |
|||