Human vs Bicolor damselfish LastZ results
Human (Homo sapiens, GRCh38) and Bicolor damselfish (Stegastes partitus, Stegastes_partitus-1.0.2) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 94. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Bicolor damselfish |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 10,100,000 | |
| Masking options |
Statistics over 251,998 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 3,064,218,037 out of 3,099,750,718 |
Uncovered: 17,381,896 out of 38,571,754 |
| Bicolor damselfish |
Uncovered: 766,554,888 out of 800,491,834 |
Uncovered: 12,688,752 out of 36,236,516 |
Block size distribution
| Size range | All 251,998 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 1,562 |
6.9 kb |
|||
| 10 bp - 100 bp | 50,876 |
3.8 Mb |
15,692 |
1.2 Mb |
|
| 100 bp - 1 kb | 197,318 |
39.4 Mb |
83,314 |
24.9 Mb |
|
| 1 kb - 10 kb | 2,230 |
3.8 Mb |
9,965 |
19.9 Mb |
|
| 10 kb - 100 kb | 11 |
151.5 kb |
76 |
1.1 Mb |
|
| 100 kb - 1 Mb | 1 |
134.8 kb |
3 |
340.3 kb |
|