Human vs Mouse Lemur LastZ results
Human (Homo sapiens, GRCh38) and Mouse Lemur (Microcebus murinus, Mmur_3.0) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 91. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Mouse Lemur |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 10,100,000 | |
| Masking options | {default_soft_masking => 1} | {default_soft_masking => 1} |
Statistics over 2,223,849 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 1,325,078,141 out of 3,099,750,718 |
Uncovered: 1,235,065 out of 38,571,754 |
| Mouse Lemur |
Uncovered: 829,559,044 out of 2,487,409,138 |
Uncovered: 1,248,793 out of 32,222,963 |
Block size distribution
| Size range | All 2,223,849 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 78,572 |
528.2 kb |
|||
| 10 bp - 100 bp | 323,250 |
15.5 Mb |
34,464 |
2.3 Mb |
|
| 100 bp - 1 kb | 1,220,295 |
526.7 Mb |
125,265 |
41.7 Mb |
|
| 1 kb - 10 kb | 599,655 |
1.3 Gb |
23,792 |
56.3 Mb |
|
| 10 kb - 100 kb | 2,076 |
26.8 Mb |
2,086 |
55.4 Mb |
|
| 100 kb - 1 Mb | 1 |
140.0 kb |
277 |
78.5 Mb |
|
| 1 Mb - 10 Mb | 119 |
438.4 Mb |
|||
| 10 Mb - 100 Mb | 49 |
1.2 Gb |
|||